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KMID : 0379119890170030119
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Korean Journal of Mycology
1989 Volume.17 No. 3 p.119 ~ p.123
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Interorder Protoplast Fusion between Pleurotus ostreatus and Ganoderma applanatum
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박용환/Park, Yong Hwan
장권열/유영복/차동열/송문태/유창현/고승주/Chang, Kwon Yawl/Yoo, Young Bok/Cha, Dong Yeul/Song, Moon Tae/You, Chang Hyun/Go, Seung Joo
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Abstract
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Interorder heterokaryons were obtained by polyethylene glycol induced fusion of protoplasts from auxotrophic mutants of Pleurotus ostreatus in agaricales and Ganoderma applanaturm in aphyllophorales. When transferred to MMM plates, all fusion colonies exhibited an extremely slow growth rate. During three times subcultivation on MCM the growth rate of fusants showed faster little by little. Seventy-five % fusion products of potoplasts showed mixed morphologies between those of P. ostreatus and G. applannatum in the first subcultivation on MCM and MGM. The phenotype of these fusanta changed similar those of P. osteatus type after three times subcultivation on MCM. However, phenotype of 25% stable strains did not change on subcultivation. Hyphae of all fusion products did not form true clamp connection. All these types did not produce primordia. A comparrison of interorder somatic hybrids was made using isozyme analysis of esterase, malate dehydrogenase and peroxidase. In most cases the enzyme patterns of G, applanat um were not distinct, however, fusant showed non-parental bands.
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